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KMID : 0829220100340010027
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Korean Journal of Oral and Maxillofacial Pathology
2010 Volume.34 No. 1 p.27 ~ p.35
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Establishment of CDK4/hTERT-Transfected HPV16 E6/E7 Immortalized Cell Lines
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Park Young-Jin
Hong Doo-Pyo
Kim Eun-Cheol
Oh Bong-Kyeong
Kim Jin
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Abstract
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Human papillomavirus (HPV) has been classified as one of the causing factors of head and neck squamous cell carcinoma (HNSCC). However, little is known about HPV-related carcinogenesis in HNSCC. The purpose of this study is to characterize immortalized human oral keratinocyte (IHOK) transfected by HPV16 E6/E7, IHOK/hcdk4 (IHOK transfected by pLXRN-hcdk4) and IHOK/hcdk4/hTERT (IHOK transfected by pLPC-hTERT-hcdk4) to reconstitute HNSCC in vitro. Conclusively, we established a new immortalized cell lines, IHOK/hcdk4 and IHOK/hcdk4/hTERT, to understand multistep carcinogenic process of oncogenic HPV16 E6/E7 in HNSCC.
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KEYWORD
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hcdk4, HPV16 E6/E7, hTERT, Immortalization
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